Dried blood spots : applications and techniques /
This book covers the fundamentals, experimental procedures, and applications of dried blood spot (DBS) sampling in combination with various qualitative and quantitative analytical techniques. This includes sample collection, storage, transportation, and sample preparation for various analysis. Exper...
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| Other Authors: | , |
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| Format: | Electronic eBook |
| Language: | English |
| Published: |
Hoboken, New Jersey :
John Wiley & Sons, Inc.,
[2014]
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| Subjects: | |
| Online Access: |
Full text (Emmanuel users only) |
Table of Contents:
- Series; Titlepage; Copyright; PREFACE; CONTRIBUTORS; PART I HISTORY, APPLICATIONS, AND HEALTHCARE; 1 Overview of the History and Applications of Dried Blood Samples; 1.1 History; 1.2 Historical Applications; 1.3 Overall Advantages and Disadvantages of Dried-Blood Spot Sampling; 1.4 Conclusion; Notes; References; 2 Dried Blood Spot Cards; 2.1 Introduction; 2.2 Filter paper; 2.3 Filter Paper Sources; 2.4 Guidelines and Printing; 2.5 Printing Adaptations; 2.6 Further Innovations in Manufacturing; 2.7 Filter Paper Cards Construction; 2.8 Wraparound Cover and Cassette Construction.
- 2.9 Filter paper Pre-Treatment2.10 The future of the Filter Paper Card; References; 3 Dried Blood Spot Sample Collection, Storage, and Transportation; 3.1 Introduction; 3.2 General Procedures and Precautions for DBS Sample Collection; 3.3 Sample storage and transportation; 3.4 Common errors in sample collection, storage, and shipment; 3.5 Conclusions; Disclaimer; References; 4 Dried Blood Spot Specimens for Polymerase Chain Reaction in Molecular Diagnostics and Public Health Surveillance; 4.1 Introduction; 4.2 Pioneering DBS Nucleic Acid Extraction Methods for PCR.
- 4.3 Direct PCR Amplification of Targeted Genomic DNA from DBS4.4 Expansion of DBS-PCR from Newborn Screening to Diagnosis of Infectious and other Diseases; 4.5 Application of DBS-PCR in Sequencing-Based Molecular Epidemiology Analyses; 4.6 Application of DBS-PCR for HIV Drug Resistance Surveillance and Monitoring in Resource-Limited Settings; 4.7 Quantitation of Infectious Agent Particles with DBS-PCR; 4.8 Conclusions; References; 5 Application of Enzyme Immunoassay Methods Using Dried Blood Spot Specimens; 5.1 Introduction; 5.2 Overview of Basic ELISA Systems.
- 5.3 Applications of ELISA Methods Using DBS5.4 Optimization and ELISA methods validation using DBS specimens; 5.5 Factors influencing the Performance of DBS-based ELISA Methods; 5.6 Conclusions; Disclaimer; References; 6 Applications of Dried Blood Spots in Newborn and Metabolic Screening; 6.1 Introduction; 6.2 Fundamentals of Dried Blood Spots; 6.3 Analytical Methods in Newborn Screening; 6.4 Detection of Metabolic Disease in a DBS; 6.5 Newborn Screening as a Subset of Metabolic Screening; 6.6 Prognostications and Predictions; Disclaimer; References.
- 7 Dried Blood Spots for Use in HIV-Related Epidemiological Studies in Resource-Limited Settings7.1 Background; 7.2 Initial Studies Describing HIV Detection in Dried Blood Spots; 7.3 Prevention of Mother-to-Child HIV Transmission (PMTCT) and Early Diagnosis of HIV Infection Among Infants; 7.4 Emerging Strategies and Applications of DBS in HIV-Related Epidemiological Studies in Resource-Limited Settings; 7.5 Conclusion; Disclaimer; Notes; References; 8 Use of Dried Blood Spot Samples in HCV-, HBV-, and Influenza-Related Epidemiological Studies; 8.1 Hepatitis C Virus; 8.2 Hepatitis B Virus.